

Dof specifically bind AAAG sequences of plant gene promoters with the Dof DNA-binding domain. The Dof (DNA-binding one zinc finger) is a plant-specific transcription factor having multiple roles such as carbon assimilation, light-mediated regulation, seed maturation and germination. Transcription factors play a very vital role in gene regulation at transcriptional level. Overall, our results could provide valuable information for better understanding the evolution of cotton Dof genes, and lays a foundation for future investigation in cotton. Analysis of RNA-Seq data indicated that cotton Dof gene expression levels varied across different tissues and in response to different abiotic stress. Genome comparison revealed that segmental duplication may have played crucial roles in the expansion of the cotton Dof gene family, and tandem duplication also played a minor role. Those genes were unevenly distributed across 13/26 chromosomes of the cotton. 115, 116, 55 and 56 Dof genes were identified respectively and all of the genes contain a sequence encoding the Dof DNA-binding domain. In this study, we performed a genome-wide screening and characterization of the Dof gene family within two tetraploid species Gossypium barbadense, Gossypium hirsutum, and two diploid species Gossypium arboreum, Gossypium raimondii.
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